Lead and cadmium induced alterations of cellular functions in leaves of Alocasia macrorrhiza L. Schott
发表在:Ecotoxicology and En 73 1238-1245    于:2010

Oxidative stress,Lead,Cadmium,Alocasia macrorrhiza,Histochemical localisation,Chlorophyll fluorescenceimage


Alocasiamacrorrhiza is afastgrowingandpropagatingherbaceousspeciescommonlyfoundin South China.TodetermineitsphysiologicalresponsestoPbandCdstresses,thebiochemical, histo chemical and cytochemical changes under PbAC2 and CdCl2 phytotoxicity were detected using leaf discsas an experimental model.After leaf discs were infiltrated in different concentrations of PbAC2 and CdCl2 solutions(0,50,100,150,200 mM) for72h,theformationofreactiveoxygenspecies (H2O2 and O2) in plant tissue were found to be exaggerated together with elevated dOH concentration and celldeath.Changesinchlorophyllfluorescence(Fv/Fm, FPSII, qP and NPQ)imagingcolours/areasof leaf discsindicateddecreasedphotosystemIIfunctionsbybothheavymetaltreatmentsandpositive reactions of antioxidants under Pb2+ stress. Results showed that fluorescent detection of hydroxylated terephthlateus ingterephthalicacidas dOH trapisasimple,yetvaluableandspecificmethodfor monitoring dOH generation in plant tissue under heavy metal stresses. AscomparedwithCd2+, Pb2+ was foundtobelesstoxic,indicatingthat A. macrorrhiza tissuemighthaveapotentialtolerance to Pb.

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